KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
The acquisition of the monoclonal antibody gene sequence of hybridoma cells is the basis for recombinant antibodies and the development of biosimilar antibodies. KMD Bioscience provides antibody sequencing services with accuracy and speed. For your convenience and to let you rest assured, KMD Bioscience follows strict rules and is conscientious and rigorous in the implementation of our services, ensuring that the antibody of interest can be accurately reconstituted before it enters downstream applications.
In the process of preparing recombinant antibodies, after the customer supplies the hybridoma cells, there are two heavy chain and two light chain problems, which leads to the diversity of subsequent light and heavy chain pairing combinations, preparing recombinant antibodies time-consuming and labor-intensive. But KMD Bioscience knows how valuable your time is, and our scientific team has finally found a solution. We designed a series of IgG primers for myeloma cells, combined with cell subcloning technology to finally obtain sequence information that is highly consistent with the target antibody.
Hybridoma Sequencing Service
--Standard monoclonal antibody sequencing services: antibody variable region VH and VL sequencing, antibody VH, VL and leader region sequencing, whole antibody VH, VL, leader and constant region sequencing, sequencing services completed in 3-4 weeks.
--Rapid monoclonal antibody sequencing service: The antibody sequence rapidly recognized from the target cell line can be obtained in 10 days.
--Other Sequencing Methods: The methods of DNA sequencing include Sanger sequencing and next-generation sequencing, Simultaneous sequencing methods including gene sequencing, long-read sequencing, whole genome sequencing, Illumina sequencing, de novo sequencing, etc.
Hybridoma Sequencing Service Workflow
|
Step |
Contents |
Timeline |
|
1 |
* Hybridoma cells expended culture and antibody titer tested by ELISA |
1-2 weeks |
|
2 |
* Total RNA extracted after Trizol cleavage |
1-2 days |
|
3 |
* Reverse transcription preparation of cDNA by RT-PCR |
1-2 days |
|
4 |
* PCR amplified the full-length gene [VHCH123 and VKCK/VLCL] genes to ensure the sequence fidelity of the variable regions FR1 and FR4. |
1week |
|
5 |
* The T vector was sequenced, the sequencing results, and the correct clone. |
3-5 days |
Hybridoma Sequencing Service Highlights
--Using high-fidelity polymerase to obtain all sequence information of monoclonal antibody genes
--Personalized monoclonal antibody sequencing
--Multi-species hybridoma sequencing: mice, rats, rabbits, etc
--Complete and rigorous bioinformatics analysis
--Customer has complete intellectual property and information security (high confidentiality)
--IgG whole gene cloning
Sample Requirements
--Customers can provide antigen and hybridoma cell lines directly
--Customers can provide antigens, hybridoma cell culture supernatants, and TriZol lysates of hybridoma cell lines (usually 5 x 106 cells / 2 ml Trizol)
--Shipping condition: communicate with our technical support in advance
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
