KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
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KMD Bioscience has built up a mature antibody production platform with our well-established antibody engineering technology. Based on our mature antibody production system and advanced polyclonal antibodies technology, we can offer comprehensive polyclonal antibody production services including the production of polyclonal antibodies in mice, rats, rabbits, and other species. Meanwhile, we can accept various antigens designed for polyclonal antibody production, such as small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins, etc. |
KMD Bioscience has experienced immunology experts, standard operating animal immunization procedures, and a standard animal facility, which can provide high-quality Polyclonal antibody products and polyclonal antibody production services to scientists and researchers. We promise to provide high-performance donkey polyclonal antibody production services and related testing services. Moreover, we ensure the ELISA titer of the positive antisera & antibody can reach 1:50,000 and ensure the western blot results for protein antigens.
KMD Bioscience can provide antibody design services to design antibodies with high specificity, affinity, and stability for its target antigens. In addition, we also provide one-stop services for polyclonal antibody production to meet the scientific research needs of global customers, such as antibody conjugation, antibody labeling, high-throughput sequencing, and antibody purification. We also provide customers with antibody-drug conjugation design solutions and design exclusive customized solutions for customers.
Donkey Polyclonal Antibody Production Service Process
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Donkey Polyclonal Antibody Production Service Workflow
|
Procedure |
Description |
Timeline |
|
Animal Immunization |
* Clients provide the antigens (small molecules, peptides, proteins); Purity>85%, concentration>0.5mg/mL, ~2mg * Immunize one donkey, 5 boosts, ELISA titer determination * Negative control: pre-immune serum collection before the first immunization, 1mL * Serum harvest and ELISA titer determination * Deliver ELISA report, 1mL pre-immune serum and 250-500mL antisera to investigators |
12-14 weeks |
|
Consecutive bleed |
* Inject 500ug antigen mixed with 0.5mL Freund's incomplete adjuvant per donkey * 100-150mL blood is collected every two weeks and ELISA titer determination * Ship the product & report to an investigator to test the serum and determine the antibodies' quality |
2-3 weeks/phase |
|
Total bleed |
* Collect all the blood and the donkey is humanely euthanized * Serum collection, provide the products according to the investigator's requirements |
1-2 weeks |
|
Antibody Purification |
* Purify the donkey sera by DEAE or affinity chromatography * Deliver the purified polyclonal antibody to investigators |
1 week |
Besides, we can provide the following antibody services:
--Antibody Identification: ELISA, WB, IF, IP, IHC, SPR, etc.
--Antibody Modification: Enzyme modification (HRP, AP, biotinylation), fluorescent labeling (FITC, FAM, etc.), fusion label (GST, His), magnetic beads labeling, quantum dot labeling, etc.
Deliverable
|
Materials |
Description |
|
Serum |
* 1mL pre-immune serum and 250-500mL antisera |
|
Antibody |
* DEAE purified antibody: 20-40mg (10-20mg purified antibody will be delivered if the investigator chooses antigen affinity purification) |
|
Report |
* Immunogen sequence, SDS-PAGE result, expression & clone sequencing report, ELISA titer result * According to the investigator's requirements, we can also provide the validation result of WB, IHC, IP, IF |
Donkey Polyclonal Antibody Production Service Highlights
--Rich experience in antibody design and polyclonal antibody production
--High affinity and specificity polyclonal antibodies
--Large-scale polyclonal antibody production capability
--Strict quality assurance: cGMP management rules
--Guaranteed positive western blot result and high ELISA titer
--A variety of antibody purification techniques are available
--One-stop services, including antigen design, animal immunization, antibody development, antibody purification, antibody labeling, high-through sequencing, titer determination, and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
