KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
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Polyclonal antibodies can recognize and bind to different epitopes on a single antigen. We have built up a mature antibody discovery platform, and have rich experience in polyclonal antibody development. With our mature antibody discovery platform and well-established antibody discovery technology, KMD Bioscience can offer comprehensive polyclonal antibody development services with a variety of different animals, including New Zealand white rabbits, goats, sheep, guinea pigs, chickens, rats, mice, and donkeys. |
The rabbit polyclonal antibody development services provided by KMD Bioscience are high-quality and affordable, supported by our mature antibody discovery platform and advanced antibody discovery technologies. KMD Bioscience is constantly refining its technologies to provide you with better polyclonal antibody development services. All of Our customized polyclonal antibodies are quality-assured, KMD Bioscience can provide a full animal immunization protocol from antigen design to Rabbit immunization and ensure 6x104 of the ELISA titer. KMD Bioscience can provide upstream and downstream services related to antibody discovery, including animal immunization, serum testing, antibody discovery, antibody purification, antibody labeling, antibody sequencing, antibody validation, antibody stability testing, and other services, providing customers with one-stop solutions for antibody development problems.
Rabbit Polyclonal Antibody Production Background
Why choose rabbits as a species for polyclonal antibody production? Choosing New Zealand white rabbits for the production of rabbit polyclonal antibodies has the following advantages: high-quality and high-affinity antibodies can be produced from anti-serum, easy rabbit immunization and serum testing for potency, high immune response, and sufficient blood volume, these advantages make the production of polyclonal antibodies convenient, fast, and cost-effective. When using rabbits as immune animals, to prevent pathogen-induced mortality, it is best to choose SPF-grade animals, and it is best to choose young female adult animals. In addition to strong immune responses, female animals are more gentle, and immunization and blood collection are more convenient. For rabbits as immune animals, the antigen requirement is roughly 50-1000ug.
Rabbit Polyclonal Antibody Production Service
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Project Name |
Contents |
Timeline |
|
ELISA guaranteed pAb production |
* Immunization of 2 New Zealand white rabbits, antisera collection, and protein A purified * ELISA titer> 1:60,000 |
8-10 weeks |
|
WB guaranteed pAb production |
* Immunization of 4 New Zealand white rabbits, antisera collection, and protein A purified * WB testing by the investigator |
10-12 weeks |
|
IHC guaranteed pAb production |
* Immunization of 4 New Zealand white rabbits, antisera collection, and protein A purified * IHC testing by investigator |
10-12 weeks |
|
Phospho-specific guaranteed pAb production |
* Immunization of 5 New Zealand white rabbits, antisera collection, and protein A purified, phosphorylated titer/non-phosphorylated titer>8:1 * Testing by investigator |
12-14 weeks |
|
DNA methylation guaranteed pAb production |
* Immunization of 5 New Zealand white rabbits, antisera collection, and protein A purified * Testing by investigator |
12-14 weeks |
|
Other guaranteed pAb production |
* Besides the above services, we can also provide Acetylation, IP, and CHIP guaranteed pAb production services
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Rabbit Polyclonal Antibody Production Service Process
Rabbit Polyclonal Antibody Production Service Workflow from Different Antigen
|
|
Protein |
Peptide |
Phospho-peptide |
|
Peptide synthesis |
3-5mg Purity>90% M.W.> 10 kDa |
~10mg >10 amino acids Purity>90% |
~10mg >10 amino acids Purity>90% |
|
Peptide conjugation |
|
KLH, BSA, OVA |
|
|
Immunization and sera collection |
Yes |
Yes |
Yes |
|
Titration by |
ELISA, WB (Optional: IF, IP, IHC) |
ELISA, WB (Optional: IF, IP, IHC) |
ELISA, WB (Optional: IF, IP, IHC) |
|
Protein-A purification |
Yes |
Yes |
|
|
Antigen-affinity purification |
Optional |
Optional |
Yes |
|
Antibody modification(Optional) |
* Enzyme modification: HRP, AP, biotinylation * Labeling Services: FITC, FAM, Magnetic beads, Quantum Dot * Fusion tags: GST, His |
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Deliverable
|
|
Contents |
|
Purified Antibody |
* 5~15 mg Protein G/A purified antibody |
|
Report |
* Immunogen sequence, SDS-PAGE, ELISA titer result * WB, IHC, IP, IF result (Optional) |
Rabbit Polyclonal Antibody Production Service Highlights
--Rich experience in polyclonal antibody development
--High affinity and specificity
--Large-scale polyclonal antibody development capability
--Ideal source of therapeutic antibody for antibody therapy
--Strict quality assurance: cGMP management rules
--Guaranteed positive western blot result and high ELISA titer
--A variety of antibody purification techniques are available
--One-stop polyclonal antibody development services, including antigen production, animal immunization, antibody discovery, antibody purification, titer determination, antibody labeling, antibody sequencing, antibody stability testing, and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
