Nucleic Acid Aptamer Synthesis Service

KMD Bioscience, with years of research experience in the field of aptamer development, has successfully built a comprehensive aptamer library system and accumulated profound project practical knowledge in the process. KMD Bioscience can provide customers with excellent aptamer library construction services and support subsequent research processes such as aptamer screening, nucleic acid aptamer sequence synthesis, functional validation, and aptamer development for specific molecules. KMD Bioscience has achieved sequence optimization, upgrading, and significant functional enhancement through professional nucleic acid adapter sequence synthesis services. With the help of an advanced adapter library construction platform, KMD Bioscience ensures that customers can obtain efficient and accurate nucleic acid adapter results. In addition, KMD Bioscience also has the ability to provide comprehensive aptamer library design services, covering the entire process from gene analysis synthesis, in vitro screening of aptamers, aptamer synthesis to affinity determination, striving to meet the diverse project needs of various customers.

The nucleic acid aptamer library constructed by KMD Bioscience covers a wide range of sequences and has a broad coverage. The core elements such as sequence length, GC ratio, and secondary structure in the library have been optimized to enrich the diversity of aptamers while strictly monitoring their quality. Based on years of experience in aptamer development, KMD Bioscience has conducted in-depth analysis of the screened nucleic acid aptamer sequences, extracted the common structural characteristics of these sequences, and continuously optimized sequence design by introducing mutations, adjusting sequence length, and other means to enhance the binding efficiency, stability, and biological activity of the aptamers. The adapter library capacity of KMD Bioscience is approximately 10 ^ 13 to 10 ^ 14, and can even reach the level of 10 ^ 14. With this high-capacity library and diverse screening techniques such as magnetic bead SELEX screening, cell SELEX screening, capture SELEX screening, etc., KMD Bioscience can efficiently screen for highly specific adapter sequences targeting specific targets from various samples such as proteins, small molecules, cells, and bacteria. These sequences include RNA aptamers, DNA aptamers, and oligonucleotides. Based on the obtained sequence, nucleic acid aptamer sequence synthesis is carried out, and the synthesized nucleic acid aptamer exhibits binding affinity at the nM to pM level, especially for protein targets, which can reach up to pM level. Customers only need to clarify their experimental requirements, and KMD Bioscience's research team will tailor a solution to fully assist them in Aptamer development research exploration.

Nucleic Acid Aptamer Synthesis Service

Nucleic acid aptamers exhibit exceptional specificity in recognizing and binding to their target molecules, allowing them to pinpoint targets amidst a myriad of molecules, effectively sidestepping the disruptions posed by nonspecific binding. Their binding capacity with target molecules is robust, sometimes even surpassing that of antibodies. This superior affinity renders nucleic acid aptamers exceptionally sensitive and precise in applications like detection and diagnosis. SELEX technology offers a highly efficient and targeted approach for the screening of nucleic acid aptamers, thereby advancing their utilization and progress across diverse domains. KMD Bioscience employs this SELEX technology to identify aptamers with strong affinity for specific, targeted substances from a randomly generated library of single-stranded nucleic acid aptamers.

KMD Bioscience uses advanced SELEX screening technology to accurately extract oligonucleotides with high affinity for targets from a large random library. After multiple rounds of screening cycles, SELEX fragments were sequenced based on their enrichment levels to obtain adapter sequences (RNA aptamers, DNA aptamers). Among them, the oligonucleotide library has been uniquely designed with fixed sequences at both ends and cleverly inserted with random sequences (RNA aptamers, DNA aptamers) in the middle. In the fixed sequence section, primer binding sites that are conducive to PCR amplification are specifically embedded. Random sequences, typically composed of 30 to 60 nucleotides, ensure high diversity in the library and enable effective binding of these sequences to target substances. The initial stage of library design plays a decisive role in screening effectiveness. The design of the fixed region must prevent self dimerization during amplification, therefore, the length of the primer region is precisely controlled between 18 and 21 nucleotides. Random regions are generally located within a length range of 30 to 60 nucleotides (nt), with 40 nt being a commonly used length. A shorter random region design facilitates subsequent truncation and application, while longer regions are more likely to screen for structurally complex and highly specific adapter sequences in adapter screening techniques. Single nucleotide molecules can dock and recognize high affinity binding sites on target molecules, and the resulting nucleotides can be assembled into short fragments as binding units for nucleic acid aptamers. A stable unit based on a thermally stable secondary structure (such as a mini DNA hairpin structure) is assembled together with a binding unit to form a full-length adapter. The selection of fixation medium is crucial in the process of ligand screening. Common fixed media include nitrocellulose membrane, gel column, microporous plate, etc. The properties of the target will affect the screening and design of aptamers. For example, for targets such as cells, bacteria, or viruses, the Cell SELEX method is often used for screening. For soluble small molecule targets, solid-phase adsorption and elution techniques or FluMag SELEX methods may be used. The selected and designed aptamers need to be validated and optimized to ensure their high affinity and specificity. Common validation methods include BLI, SPR measurement, etc. The detailed process of constructing libraries and screening aptamers at KMD Bioscience is shown in Figure 1.

Figure 1 Screening process diagram for nucleic acid aptamers

Nucleic Acid Aptamer Synthesis Service Workflow

Nucleic Acid Aptamer Synthesis Service Advantages

✔ The library has a capacity of 10 ^ 13-10 ^ 14, sufficient to screen for nucleic acid aptamers targeting customer targets.

✔ SELEX technology platform mature: the affinity of nucleic acid aptamers obtained through screening can reach the nM-pM level.

✔ Traceability of experimental records: QC quality control standards, Chinese and English experimental reports, original experimental records.

✔ One on one personalized solution customization (including screening and subsequent verification plans, etc.) to meet the research project needs of various clients.

✔6-10 rounds of pressure screening can obtain high affinity and high specificity nucleic acid aptamers.

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