KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Phage display technology utilizes a phage-based display library to identify and develop particular protein and antibody interactions, and is now one of the most potent and widely used laboratory technologies for the study of antibody discovery. KMD Bioscience specializes in advanced phage display technologies for a variety of service projects. With the mature antibody production platform we have built up, we can offer high-quality phage display library construction and custom phage display library screening services to meet various customer needs. KMD Bioscience now offers multi-species monoclonal antibody production services, covering human, rat, rabbit, chicken, sheep, goose, pig, bovine, horse, donkey, camel, alpaca, etc. With skilled technology, our scientists can offer high-quality phage display library construction and custom antibody library screening services to meet our clients’ different requirements.
Phage display service has many applications in drug development, such as identifying new drug targets, designing new therapeutic proteins, and developing vaccines for infectious diseases. It is also used in research applications to study protein-protein interactions, protein-DNA interactions, and protein-ligand interactions. KMD Bioscience is devoted to providing one-stop technical services including protein expression, affinity analysis, antibody humanization, antibody affinity maturation and related services to save you time. With the time saved, you can achieve more in your research. Backed by a wealth of experience and advanced platforms, KMD Bioscience can provide comprehensive phage display library construction services.
Antibody Phage Display Library Platform:
Phage display technology (PDT) is a molecular diversity technology that allows the presentation of large peptide and protein libraries on the surface of filamentous phage. In 1985, Smith, G.P. proposed inserting exogenous genes into the modified phage shell protein and expressing fusion proteins containing exogenous proteins or polypeptides, called fusion phages. Through repeated adsorption-elution-amplification processes, phages that specifically bind to target proteins are screened from phage libraries. Then enrichment, amplification and gene sequencing were performed to infer the amino acid composition of exogenous proteins. A crucial advantage of this technology is the direct link between the experimental phenotype and its encapsulated genotype, which allows the evolution of the selected binders into optimized molecules. It has been successfully applied to epitope analysis, monoclonal antibody screening, determination of protein functional antagonist polypeptides or mimetic peptides and plays an essential role in drug screening, vaccine design, etc.
Figure 1 Diagram of phage display technology
Technical Process:
In general, filamentous bacteriophages (e.g. M13 phage) are the most popular choice for phage display and are extensively used in many research fields. Using M13 filamentous phage shell protein pIII and PVIII display technologies, KMD Bioscience can construct different kinds of natural or immune phage antibody display libraries for our clients, and the library capacity can reach 1011.
Specific polypeptides can be efficiently screened by the affinity between displayed polypeptides and target proteins (mainly refers to antibodies, antigens, some growth factor receptors, etc.) or other biological macromolecules. First, the target protein is solidified, e.g. by binding to a plastic plate (96-well plate), then the phage libraries are added and reacted with it. After washing, the unbound phages were washed out and the affinity phages were captured. The captured phages were eluted and then infected with E. coli for further amplification. Such an "adsorption-elution-amplification" enrichment process is called panning. A round of panning can enrich 103 folds of the phages. Typically, short peptides interacting with target proteins can be screened from the phage libraries of 109-1010 after 3-4 rounds of panning.
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Figure 2 Workflow of phage display technology
Platform Highlights:
--Customized Library Design: The libraries are designed based on the target molecule of interest, the screening method, and the desired properties of the selected candidates.
--Library Construction: Phage libraries provided by KMD Bioscience are constructed using high-throughput cloning techniques to ensure a diverse and representative library. The libraries are also validated to ensure the correct insert size and quality.
--Biopanning and Selection: Phage display services use various biopanning and selection strategies to isolate phages that bind to the target molecule with high affinity and specificity. The selected phages are then amplified and sequenced for downstream analysis.
--Downstream Analysis: After biopanning and selection, phage display services perform downstream analysis to identify the binding peptides or proteins. This includes sequencing, bioinformatics analysis, and validation of the binding affinity and specificity (SPR and BLI).
--Optimization and Cost-saving Plan: KMD Bioscience also offers optimization services to improve the binding affinity and specificity of the selected phages. This includes mutagenesis, affinity maturation, and optimization of the screening conditions.
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
