Antibody Platform

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Recombinant Mouse Monoclonal Antibody Production Service

KMD Bioscience has been working on antibody production research for many years. Supported by a skilled team of scientists and standard operating procedures, KMD Bioscience can provide comprehensive recombinant monoclonal antibody production services to you. Mouse monoclonal antibody has many advantages such as high specificity, stable passage and extensive applications in basic research, medical diagnosis and tumor therapy. The monoclonal antibody can be produced via traditional hybridoma technology or phage display technology. With extensive experience in the field of phage display technology, our scientists can construct high-quality immunized mouse antibody libraries and screen out high-affinity monoclonal mouse antibodies.  

KMD Bioscience provides high-quality monoclonal antibody production services covering antigen design, synthesis and modification, animal immunization, phage library construction and screening to antibody modification. Meanwhile, KMD Bioscience can develop monoclonal antibodies from various host species, including rat, rabbit, camel and other species.

 

Recombinant Mouse Monoclonal Antibody Generation:

 

KMD Bioscience offers two production methods for mouse monoclonal antibodies: recombinant monoclonal antibody production with phage display technology; hybridoma cell line production with cell fusion technology.

In the phage technique for the development of recombinant mouse monoclonal antibody, we cloned the variable regions of the murine antibody (Vλ, Vk, and VH) into the phagemid system with the M13 phage pIII fusion protein, subsequently we cloned the screened high specificity and high-affinity rabbit-derived antibody variable regions (Vλ, Vk, and VH) into plasmid vectors carrying mouse antibody scaffold proteins and transiently expressed in our recombinant expression platform (up to ~5 g/L).

 

Service Process:

 

 

Service Content:

 

Procedure

Carrier Type

Deliverable

Lead Time

Hapten Modification

/

Modified small molecule derivatives

LC-MS result

3-4 weeks

Complete Antigen Production

* Ovalbumin (OVA)

* Keyhole limpet hemocyanin (KLH)

* Human Serum Albumin (HSA)

* Human gamma globulins

Complete antigen with SDS-PAGE, UV or MALDI-TOF identification results

1-2 weeks

Phage Antibody Library Production

* Antigen emulsification

* Immunization

* Collection of splenocytes (or peripheral blood lymphocytes), variable region gene isolation

* Phagemid construction, transfection expression, library construction

* Antibody screening, re-cloning, antibody expression

PCR results, immunoassay results (including ELISA titer and specificity test)

1-2 months

Recombinant antibody production

* Antibody screening, construction of expression plasmid, eukaryotic or prokaryotic expression of recombinant antibodies

* Recombinant Antibody, expression plasmid, cloned strain, experimental report

1-2 months

 

Service Highlights:

 

--Rich experience in recombinant monoclonal antibody development

--High affinity and specificity

--Sequence diversity

--Multiple antibody expression validation options are available

--Antibody sequence humanization service is available

--Mature antibody and recombinant protein platform

--Strict quality assurance: cGMP management rules

--Guaranteed positive western blot result

--A variety of antibody purification techniques are available

--One-stop services, including antigen preparation, antibody development, phage library construction, antibody modification and related services

 

How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806;