KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
KMD Bioscience has been working on monoclonal antibody development research for many years. Supported by a skilled team of scientists and standard operating procedures, KMD Bioscience can provide comprehensive recombinant antibody expression service for global customers. Mouse monoclonal antibody has many advantages such as high specificity, stable passage, and extensive applications in basic research, medical diagnosis, and tumor therapy. The monoclonal antibody can be produced via traditional hybridoma technology or recombinant monoclonal antibody technology. With extensive experience in phage display technology, our scientists can construct high-quality immunized mouse antibody libraries and screen out high-affinity mouse recombinant antibodies.
KMD Bioscience provides high-quality recombinant antibody expression services covering antigen design, synthesis and modification, animal immunization, phage library construction, antibody screening, antibody modification, antibody validation, and antibody sequencing. Meanwhile, KMD Bioscience can develop monoclonal antibodies from various host species, including rats, rabbits, camels, and other species.
Recombinant Mouse Monoclonal Antibody Development
KMD Bioscience offers two production methods for mouse monoclonal antibodies: recombinant monoclonal antibody production with phage display antibodies technology; and hybridoma monoclonal antibody production with cell fusion technology.
In the phage display technology for the development of recombinant mouse monoclonal antibody, we cloned the variable regions of the murine antibody (Vλ, Vk, and VH) into the phagemid system with the M13 phage pIII fusion protein, subsequently, we cloned the screened high specificity and high-affinity rabbit-derived antibody variable regions (Vλ, Vk, and VH) into plasmid vectors carrying mouse antibody scaffold proteins and transiently expressed in our recombinant expression platform (up to ~5 g/L).
Recombinant Mouse Monoclonal Antibody Development Service Process
Recombinant Mouse Monoclonal Antibody Development Service Workflow
|
Procedure |
Carrier Type |
Deliverable |
Lead Time |
|
Hapten Modification |
/ |
Modified small molecule derivatives LC-MS result |
3-4 weeks |
|
Complete Antigen Production |
* Ovalbumin (OVA) * Keyhole limpet hemocyanin (KLH) * Human Serum Albumin (HSA) * Human gamma globulins |
Complete antigen with SDS-PAGE, UV, or MALDI-TOF identification results |
1-2 weeks |
|
Phage Antibody Library Production |
* Antigen emulsification * Animal Immunization * Collection of splenocytes (or peripheral blood lymphocytes), variable region gene isolation * Phagemid construction, transfection expression, phage library construction * Antibody screening, re-cloning, recombinant antibody expression |
PCR results, immunoassay results (including ELISA titer and specificity test) |
1-2 months |
|
Recombinant Antibody Expression |
* Antibody screening, construction of expression plasmid, * * Recombinant antibody expression systems include prokaryotic, eukaryotic, and other expression systems |
* Recombinant Antibodies, expression plasmid, cloned strain, experimental report |
1-2 months |
Recombinant Mouse Monoclonal Antibody Development Service Highlights
--Rich experience in recombinant monoclonal antibody development
--High affinity and specificity
--Sequence diversity
--Multiple recombinant monoclonal antibody validation options are available
--Antibody humanization service is available
--Mature antibody and recombinant protein platform
--Strict quality assurance: cGMP management rules
--Guaranteed positive western blot result
--A variety of antibody purification techniques are available
--One-stop services, including antigen preparation, antibody development, phage library construction, antibody modification, and related services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: [email protected] or Tel: +86-400-621-6806;
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：[email protected]
Hot Line： +1-315-239-3085
Technical Support：[email protected]
