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Mouse Monoclonal Antibody Production Service


KMD Bioscience strives to offer high-quality recombinant antibody production to our customers. Mice monoclonal antibody has many features such as targeting a single epitope, high specificity, stable passage, large-scale manufacturing, and extensive applications in basic research, medical diagnosis, and tumor therapy. Backed by upgraded hybridoma monoclonal antibody technology and extensive practical experience, our scientific team has developed mice monoclonal antibodies with high sensitivity and specificity to bring a better outcome for your downstream services.

To give you more choices and save you time, KMD Bioscience is engaged in providing comprehensive mouse monoclonal antibody development services to meet your needs. At present, KMD Bioscience can provide two mouse monoclonal antibody production protocols: hybridoma monoclonal antibody technology and phage display technology. Meanwhile, under the assistance of our well-established and powerful antibody engineering platform, antigen design, peptide synthesis, gene expression, immunization, antibody purification, identification, and antibody labeling services are provided to support your research.

Hybridoma technology is an important technique for the development of monoclonal antibodies and a powerful tool for recombinant antibody production. KMD Bioscience utilizes the classic hybridoma technology of mice to produce mouse monoclonal antibodies. We can produce antibodies on a large scale from developed hybridoma cell lines and consistently provide reliable results in various applications such as enzyme-linked immunosorbent assay (ELISA), Western blotting, flow cytometry, and immunohistochemistry (IHC).


Mouse Monoclonal Antibody Development


Production of mouse monoclonal antibodies by our hybridoma technology platform: Customers can provide small molecules, polypeptides, modified polypeptides, proteins, fusion proteins, modified proteins, and antigens for monoclonal antibody production. Meanwhile, we also provide antigen customization services, including DNA synthesis, plasmid construction, protein expression, protein purification, protein modification, peptide synthesis, peptide modification, peptide and carrier protein cross-linking, small molecule structure analysis, and carrier protein conjugation. The research team at KMD Bioscience has extensive experience in developing vitro diagnostic antibodies & antigens, especially in the selection and identification of antibodies (such as in vitro detection of antigen immunogenicity). Your questions about the program, the process, etc. will be answered professionally and in detail by our scientists.

Meanwhile, our patent adjuvant (FMA01# FastAb Mouse Adjuvant) can cause a strong immune response after 21 days of immunization. In addition, we also provide a faster way- surgical spleen cell immunization to save the investigator's time.


Mouse Monoclonal Antibody Development Service Process




Mouse Monoclonal Antibody Development Service Workflow






* Immunization of 5 mice (BALB/c), 3-4 times.

* Sera collection from 5 mice and ELISA titer determination.

* The immunization report will be delivered to the investigator.

4-6 weeks

Hybridoma Monoclonal Antibody Screening

* Spleen cells (the highest serum titer) fused with mouse myeloma cells.

*  Limiting dilution of fusion cells.

* HAT selection.

* Selection of 1-10 positive clones and subcloning to 96 plates.

* Screening positive subclones by ELISA and expansion of cultivation.

* 1mL supernatant/positive clones delivered to the investigator for testing.

4-6 weeks

Production of Ascites Fluid (Optional)

* The hybridoma cells selected by the investigator were used to produce ascites, mice were immunized, Protein A/G purified the ascites, and ELISA titer determination.

*  Purified antibody is delivered to the customer, <5mg/clone.

*  Provide detailed report and residual ascites antibody.

1-3 weeks

Functional Verification (Optional)

* ELISA, WB, IF, FLAC, IHC, etc.

1-3 weeks


Mouse Monoclonal Antibody Development Service Highlights:


--Rich experience in monoclonal antibody development.

--High affinity and specificity—pM grade.

--Suitable for the production of antibodies with inactivated antigens.

--Suitable for Immunohistochemistry of formaldehyde fixation.

--Applicable to different levels of antibody screening protocols, such as epitope-specific antibody screening.

--By using our FastAb Mouse Adjuvant, the antibody titer will peak after 3rd or 4th injection, decreasing the immunization timeline and immunogen dosage.

--One-stop downstream services, including antigen production, antibody development, ELISA kit development, etc.


How to Order?

If you have any questions regarding our services or products, please feel free to contact us by E-mail: or Tel: +86-400-621-6806;