KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience has been active in antibody production for over 9 years. Backed by an experienced scientific team and advanced technology, KMD Bioscience can provide customized monoclonal antibodies of different species. Based on the complete and independent antibody discovery platform, KMD Bioscience covers the upstream and downstream services of antibody production and can realize one-stop technical services including antibody synthesis, antibody purification, antibody sequencing, antibody affinity maturation, and so on.
Sheep Monoclonal Antibody Production Background
Monoclonal sheep antibodies have great potential for biomedical, veterinary, and agricultural purposes. Sheep are mammals of the order Artiodactyla, subfamily Caprinae, and genus Sheep. Sheep polyclonal antibodies have been used as specific and high-affinity immunoprobes in various immunochemical methods. Compared with the widespread application of sheep monoclonal antibodies, the development of sheep monoclonal antibody technology is slow. Sheep monoclonal antibodies are produced by fusing sheep lymphocytes with mouse myeloma lines, but hybridoma cells cannot effectively produce them, and the secretion time of specific antibodies is short. Although traditional sheep monoclonal antibodies can be produced through improved hybridoma technology, their production efficiency is too low. Therefore, we use phage display technology instead of hybridoma technology. Antibody phage display technology has also been allowed to generate monoclonal antibody fragments from many other species, including humans, rabbits, chickens, goats, cows, camels, and others.
KMD Bioscience is a provider of mature monoclonal antibodies, we offer high-quality sheep antibody production service to our customers for distinguished research applications.
Sheep Monoclonal Antibody Production Service Process
Sheep Monoclonal Antibody Production Service Workflow
|
Steps |
Specification |
periods |
|
Step1: Sheep Immunization |
* A healthy, full-aged Sheep was immunized 5-7 times * Serum titers were measured by ELISA and collected peripheral blood |
9-10 weeks |
|
Step2: cDNA synthesis (Clients can provide cells) |
* Total RNA extraction * RT-PCR * PCR amplification with Actin-specific primers to identify the quality of cDNA |
1 day |
|
Step3: Phage Display Antibody Library Construction |
* Primer design & synthesis * PCR amplification of variable region genes of heavy using cDNA as a template * Plasmid construction & transformation: after enzyme digestion, the antibody sequence and phagemid vector were ligated and transformed into TG1 host bacteria by electric shock to construct antibody phage display libraries * Identification: 20-50 clones were randomly selected, PCR identification, sequencing, and analysis of antibody sequences |
3-4 weeks |
|
Step4: Phage Display Antibody Library Screening (3 Rounds) |
* The antigenic limit pressure method is used for 3 rounds of panning * Identification: 96 clones were randomly selected for ELISA titer determination * Up to 96 positive clones were sequenced, and the 10-15 clones were selected for prokaryotic expression transformation * 10-15 positive clones were expressed to construct antibody supernatant |
3-4 weeks |
Note:
1. If the client provides sheep peripheral blood lymphocytes, lymph node cells, or spleen cells (avoid RNA degradation, dry ice transport), the service can start from the second step to construct a phage display antibody library, only 3-4 weeks is needed.
2. Cell lysates were prepared using Trizol solution and transported on dry ice.
Deliverables
--Experimental report: including detailed construction procedures and representative sequence information
--Antibody library 3-5ml, >1⨯1013 /ml phage particles, Effective storage capacity>109
--10-15 positive clones were expressed to prepare antibody supernatant (Sequence non-repetition)
--10-15 expressing strains of positive clone (Sequence non-repetition)
Sheep Monoclonal Antibody Production Service Highlights
--The resulting antibodies have higher specificity and affinity
--Rich experience accumulation, mature technical route, stable and reliable product quality, high sensitivity
--Strict quality control system, and standardized operation process, to ensure the quality of monoclonal antibodies
--It has a perfect protein platform and a variety of expression systems for choice
--Providing mass production services of monoclonal antibodies to meet the needs of industrial production
--Sequence diversity
--Multiple antibody expression validation options are available
--Antibody humanization service is available
--Providing integrated services from antigen preparation to phage display library construction and antibody screening
--Providing antibody labeling, antibody screening, kit development, immunological testing, and other value-added services
How to Order?
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
