KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience has been working in the field of monoclonal antibodies for many years. We have experienced antibody production technicians, accumulated a large number of recombinant antibody production experience, and can provide customers with high-quality antibody products and antibody services. KMD Bioscience has established a mature antibody platform and hybridoma monoclonal antibody technology platform, which can provide customers with recombinant monoclonal antibody production services for different species, including rabbits, mice, camels, sheep, chickens, and sharks.
Guinea pigs are an ideal source of antibodies in allergic reactions and allergy studies and are also important model animals in studying various infectious diseases. KMD Bioscience provides high-quality guinea pig monoclonal antibody production services, using hybridoma monoclonal antibody or phage display technology, we can produce monoclonal antibodies with high specificity, activity, and affinity for customers quickly. Relying on the perfect antibody platform, KMD Bioscience focuses on providing one-stop technical services from antigen design, synthesis and modification, animal immunization, hybridoma cell culture or phage display antibody library construction, and screening to downstream antibody modification and application identification to meet the different needs of customers.
KMD Bioscience can provide both hybridoma technology and phage display technology to produce guinea pig monoclonal antibodies, developing guinea pig monoclonal antibodies with excellent stability and productivity. Our scientists have confidence and sufficient project experience to develop guinea pig monoclonal antibodies with high affinity and specificity.
Guinea pig monoclonal antibodies by phage display technology can be stored on granular carriers in the form of antibody genes, combined with the advantages of different protein expression systems, such as the use of cell-free expression systems, can achieve animal-free production and obtain recombinant monoclonal antibodies with high specificity and high affinity.
After years of phage display technology accumulation, we can isolate the variable region (Vλ, Vk, and VH) genes of different species, recombine these genes, and clone them into the antibody backbone protein scaffold to build a full-length antibody library. Using different screening strategies, such as antigen regression, combined with our multi-way antibody production platform for recombinant antibody expression (yield up to -5g/L), providing customers with recombinant monoclonal antibodies with high specificity, high stability, and high affinity.
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Guinea Pig Monoclonal Antibody Production Service Workflow
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Steps |
Service Content |
Delivery Materials |
Cycle |
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Hapten-modified |
Small molecule modification |
* Identification results of the modified small molecule derivative LC-MS |
3-4 weeks |
|
Complete antigen Generation |
Ovalbumin |
* Complete antigen SDS-PAGE, UV, or MALDI-TOF identification results |
1 week |
|
Keyhole cyanin |
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Human serum albumin |
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Human-γ globulin |
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Construction of phage display antibody libraries or hybridoma cell culture |
Animal immunization- guinea pig immunization: Antigen emulsification immunize 2-3 guinea pigs 5 times |
* PCR results, immunoassay results (titer and specificity results containing ELISA), experimental records |
16-18 weeks |
|
Splenocytes (or peripheral blood lymphocytes) are collected and variable zone genes are isolated |
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Phage display library construction or hybridoma monoclonal antibody production |
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Library screening (solid-phase screening, liquid-phase screening, etc.) or hybridoma screening |
* Antibody sequence, library screening results, ELISA positive clone identification results, experimental reports |
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Recombinant antibody expression |
Antibody screening, construction of expression plasmids, eukaryotic or prokaryotic expression of recombinant antibodies |
* Recombinant antibodies, expression plasmids, clonal strains, experimental reports |
1-2 months |
--High antibody titer: guinea pig monoclonal antibody production with high efficiency and sensitivity to ensure delivery and meet experimental needs
--Enables large-scale production of monoclonal antibodies
--Strict quality control system, experienced laboratory technicians, and GMP-level operation level, to ensure reliable results
--A variety of expression systems and schemes are available
--Abundant antibody expression vector resources
--Has a mature antibody and recombinant protein platform to achieve project integration
--Antigen immune diversification: Customers can provide small molecules, peptides, modified peptides, proteins, fusion proteins, modified proteins, etc. as antigens for monoclonal antibody preparation
--Service can be customized: prepared according to customer needs, customized experimental protocol for each customer, to meet the special needs of each customer
Comparison of Monoclonal Antibodies in Different Species
|
Type |
Peculiarity |
|
Murine monoclonal antibody |
* The preparation method and technology are mature, and it is a commonly used model species * Low antigen demand and low cost * Small batch-to-batch variation, fast and continuous production |
|
Rabbit monoclonal antibody |
* The preparation system is mature and the preparation cost is relatively low * Strong specificity, higher affinity and activity compared to rodents * Wider range of antigen adaptation |
|
Alpaca monoclonal antibody |
* It is a heavy-chain antibody with a wide range of antigen determinant binding functions * Due to the presence of disulfide bonds in the structure, the stability is stronger * Easier to carry out humanized transformation |
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* Larger size, suitable for larger amounts of serum or antibody preparation * High efficiency, high stability, good commercialization of products, good applicability * Wide range of applications, covering detection and treatment and other fields |
If you are interested in our services, please call us directly at +86-400-621-6806 or send an email to info@kmdbioscience.com
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com
