KMD Bioscience Co., Ltd.
400-621-6806
Service Line：+1-315-239-3085
Address：FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
KMD Bioscience has been committed to the research of immunoassay products and technologies nearly 10 years, relying on many well-known brands of ELISA kits and related products and services, we can provide high-quality ELISA technical services. Our products cover a wide range of common species such as humans, mice, rats, etc. KMD Bioscience’s ELISA kits have been validated in many ways to ensure reliable results with different sample types. Our research experts have rich experience in biopharmaceutical companies, we can complete ELISA experiments quickly to ensure that sensitive and accurate data are obtained in a short period, which saves costs and avoids wastage of reagents and samples. In addition, KMD Bioscience can develop custom service according to customers' ELISA requirements, providing complete, high-quality ELISA construction, validation, results analysis and other anylasis and testing services.
ELISA (Enzyme-linked Immunosorbent Assay), based on immunological reaction, combines the specific reaction of antigen and antibody with the efficient catalytic effect of the enzyme on the substrate. It is based on the adsorption of a known antigen or antibody onto a solid-phase vector, so that the enzyme-labeled antigen-antibody reaction can be carried out on the surface of the solid-phase, and the antigen or antibody can be measured qualitatively or quantitatively. The solid-phase vector is a polystyrene microtitre plate, and the reaction of antigen and antibody is carried out in the wells of the titer plate, and after incubation with each reagent, the excess free reactants can be removed by washing, to ensure the specificity and stability of the test results. Therefore, ELISA assay has the advantages of high sensitivity, rapid detection, high specificity and good reproducibility, and is widely used in many fields such as immunodiagnosis, detection and biology. In practice, there are many kinds of specific method steps through different experimental designs. KMD Bioscience can provide ELISA experimental services, including the sandwich method, competition method etc.
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Project |
ELISA Experiment |
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Experimental Principle |
* The principle of ELISA is based on the solid-phase solidification of antigens or antibodies and the enzymatic labeling of antigens or antibodies. In the assay, the test specimen (in which the antibody or antigen is measured) reacts with the antigen or antibody on the surface of a solid-phase vector. The antigen-antibody complex formed on the solid phase vector is separated from other substances in the liquid by washing. Enzyme-labeled antigen or antibody is then added, which also binds to the solid-phase vector by the reaction. At this point, the amount of enzyme in the solid phase is proportional to the amount of the substance being examined in the specimen. After adding the substrate to the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the substance examined in the specimen, so it can be analyzed qualitatively or quantitatively according to the shade of the color. |
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Application |
* Detects the concentration of target proteins in samples by using antigen-antibody reaction, commonly used in the detection of secreted proteins.
* The concentration of target proteins in the sample can be quantitatively calculated by using the same batch of test standards to obtain the content of target proteins in each experimental group.
* Can be used to determine intermolecular interactions, such as protein-protein and protein-nucleic acid interactions. |
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Advantage |
* Multiple experimental conditions can be measured simultaneously, and advantageous experimental conditions can be quickly selected.
* Convenient operation, can easily obtain quantitative data through repeated measurements and requires fewer reagents
* The natural conformation and optimal reaction conditions of proteins can be maintained during the experimental process so that the detection conditions are closer to the physiological state. |
Sandwich ELISA assays are commonly used to detect large molecules of antigen or antibody and are called double-antibody sandwich assays because the target is sandwiched between a capture antibody encapsulated on the well and a detection antibody. They are generally categorized as double antibody sandwich assay, which is used to detect the antigen, and double antigen sandwich assay, which is used to detect the antibody.
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Project |
Sandwich ELISA |
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Experimental Principle |
*Take the double antibody sandwich method as an example: an antibody with specificity is coated on an enzyme plate and added to the sample to be tested. If the sample contains the antigen to be tested, it will bind specifically to the antibody coated on the enzyme plate, and then another antibody directed against the antigen to be tested is added, which is usually called the detection antibody. The detection antibody is usually labeled with HRP enzyme, and then TMB, the substrate of HRP enzyme, is added. TMB is catalyzed by the enzyme and undergoes a color reaction; the more antigen in the sample, the darker the color will appear. |
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Application |
*This method is mainly applicable to the qualitative or quantitative detection of antibodies, antigens and proteins. |
Service Highlight:
--Have a large product library, with a variety of high-quality ELISA application antibodies and ELISA test kits.
--Reliable data results, batch to batch consistence.
--Provide complete test reports, including raw data, detailed analysis of the results, each experimental parameter and the model of the instrumentation used, which can be directly used for thesis writing.
--Provide competitive ELISA testing services for small molecule antigens, pesticide residues, antibiotic residues, and other traceable documents and data.
If you have any questions regarding our services or products, please feel free to contact us by E-mail: info@kmdbioscience.com or Tel: +86-400-621-6806.
FL-4, Building A5, International Enterprise Community, Tianjin, China
Email：info@kmdbioscience.com
Hot Line： +1-315-239-3085
Technical Support：sales@kmdbiosci.com